Aging Muscle Cells May Alter Neighboring Cells Through Cell-to-Cell Signals | Aging-US

Aging-US #published this #research paper on May 15, 2026, in Volume 18, titled “Extracellular vesicles released by senescent myoblasts affect recipient cells via miRNA-target interactions" by #researchers from the Exercise Metabolism Research Group, Department of Kinesiology, McMaster University, Hamilton L8S 4L8, Ontario, Canada; Department of Pediatrics, McMaster University Health Sciences Centre, Hamilton L8N3Z5, Ontario, Canada; MRC-Versus Arthritis Centre of Excellence for Musculoskeletal Ageing Research, School of Life Sciences, University of Nottingham, Nottingham NG7 2TQ, Nottinghamshire, United Kingdom. @McMasterUTV #aging #cellularsenescence #metabolism #muscle #openaccess #openscience #PaperSpotlight #peerreview #journal #publishing #meded DOI - https://doi.org/10.18632/aging.206379 Corresponding author - Gianni Parise - pariseg@mcmaster.ca Abstract Cellular senescence is a state of irreversible cell cycle arrest accompanied by a robust Senescence-Associated Secretory Phenotype (SASP) that disrupts the local and systemic microenvironment. In skeletal muscle, aging is accompanied by the dysfunction of resident muscle stem cells, also known as satellite cells, as well as chronic, low-grade systemic inflammation, which is partially driven by the SASP. Extracellular vesicles (EVs) are emerging as potential mediators of SASP intercellular communication; however, little is known within the context of skeletal muscle senescence. Here, we observed that senescent myoblasts release SASP factors in vitro that reduce myotube diameter by approximately 30% (p less than 0.05). EVs released by these cells contained miRNAs directly implicated in cellular senescence and anti-apoptotic signalling. We identified 22 differentially expressed miRNAs (FDR less than 0.05; |FC| greater than 1.5) within senescent myoblast-derived EVs (SEVs), including miR-34a/b/c and miR-22. Furthermore, miR-301a-3p was revealed as a novel mediator of several genes related to senescence. SEV transfer onto myoblasts significantly upregulated the anti-apoptotic gene Mdm2 (p less than 0.05; 60% increase), a member of the senescence-associated super-enhancer family. Lastly, we integrated the miRNA gene target data with the transcriptomic profiles of adult (8mo) and geriatric (28mo) mouse gastrocnemius muscles and observed a significant overlap in gene regulation by SEV miRNAs during aging. Taken together, these results indicate that EVs and their miRNA cargo may be potential contributors to the SASP and senescence-associated signalling in skeletal muscle during advanced aging. Sign up for free Altmetric alerts about this article - https://aging.altmetric.com/details/email_updates?id=10.18632%2Faging.206379 Subscribe for free publication alerts from Aging - https://www.aging-us.com/subscribe-to-toc-alerts Keywords - aging, cellular senescence, extracellular vesicles, myoblasts, miRNA, multi-omics To learn more about the journal, please visit https://www.Aging-US.com​​ and connect with us on social media at: Bluesky - https://bsky.app/profile/aging-us.bsky.social ResearchGate - https://www.researchgate.net/journal/Aging-1945-4589 X - https://twitter.com/AgingJrnl Facebook - https://www.facebook.com/AgingUS/ Instagram - https://www.instagram.com/agingjrnl/ LinkedIn - https://www.linkedin.com/company/aging/ Reddit - https://www.reddit.com/user/AgingUS/ Pinterest - https://www.pinterest.com/AgingUS/ YouTube - https://www.youtube.com/@Aging-US Spotify - https://open.spotify.com/show/1X4HQQgegjReaf6Mozn6Mc MEDIA@IMPACTJOURNALS.COM