DARTS Assay For Studying Rapamycin/mTOR Interaction-Preview

Watch the Full Video at https://www.jove.com/v/59656/a-semi-quantitative-drug-affinity-responsive-target-stability-darts?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022. A Semi-Quantitative Drug Affinity Responsive Target Stability (DARTS) assay for studying Rapamycin/mTOR interaction - a 2 minute Preview of the Experimental Protocol Chen Zhang, Min Cui, Yazhou Cui, Aubryanna Hettinghouse, Chuan-ju Liu New York University Medical Center, Department of Orthopaedic Surgery; New York University School of Medicine, Department of Cell Biology; In this study, we enhanced the data analysis capabilities of the DARTS experiment by monitoring the changes in protein stability and estimating the affinity of protein-ligand interactions. The interactions can be plotted into two curves: a proteolytic curve and a dose-dependence curve. We have used mTOR-rapamycin interaction as an exemplary case. Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: https://www.youtube.com/c/JoVEJournalofVisualizedExperiments