Mycelial ageing in Flammulina filiformis: physiology, metabolites and bacteria by culture age
In cultivated Flammulina filiformis strains, the abstract reports that ergosterol and catalase activity fell and yield decreased as culture age rose, with mycelial vitality good in the first 15 days and marked ageing after 60 days. [1]
Automatic summary · not editorially reviewed
The key findings
As culture age increased, ergosterol content and catalase activity declined continuously, mycelial morphology deteriorated and yield decreased. [1]
Acinetobacter dominated the mycelium-associated bacterial community from 15 to 60 days, while Serratia was predominant in the 90-day group. [1]
The authors report mycelial vitality remained good within the first 15 days of cultivation, with significant ageing after 60 days. [1]
The source at a glance
- Study type / format
- Abstract of a laboratory study combining physiological, morphological, metabolomic and microbiomic analyses
- Population / context
- Cultivated Flammulina filiformis strains of different culture ages
- Summary based on
- Study abstract
- Original publication
- September 21, 2026
Indicators measured across culture ages
The abstract describes an assessment of Flammulina filiformis strains of different ages. It combined physiological-biochemical indicators, morphological indicators, non-targeted metabolomics and microbiomics. As culture age increased, ergosterol content and catalase activity declined continuously, mycelial morphology gradually deteriorated and yield continued to decrease. The abstract does not report strain numbers, sampling intervals beyond the named time points, or statistical details.
Supporting source [1]
Metabolite and bacterial shifts with age
The abstract reports that the relative content of lipids and lipid-like molecules decreased overall, while the proportion of organic acids and their derivatives increased, and the oxidative phosphorylation pathway remained significantly enriched. The richness and diversity of the mycelium-associated bacterial community decreased significantly and its structure changed. Acinetobacter dominated from 15 to 60 days, whereas Serratia was predominant in the 90-day group. Correlation analysis linked the metabolites MG 14:1_0:0_0:0, aspartic acid-threonine-aspartamide and NAD to multiple physiological-biochemical indicators, and N-arachidonyl glycine to 21 core bacterial genera. These are correlations, not evidence of cause.
Supporting source [1]
Vitality window and proposed use
The abstract concludes that mycelial vitality of F. filiformis remains good within the first 15 days of cultivation, with significant ageing occurring after 60 days. The authors state that these findings provide a theoretical basis for optimizing fungal strain preservation techniques and regulating mycelial ageing. This is a laboratory assessment of fungal mycelium, not a study of human ageing or of any health outcome, and the abstract does not report whether the proposed preservation or regulation approaches were tested.
Supporting source [1]
How to interpret this
Abstract only; the full text was not reviewed. Strain numbers, replicate counts, sampling schedule and statistical methods are not reported in the abstract, and the correlation findings do not establish causation. Results concern fungal mycelium, not human health.
Based on the study abstract; the full paper, tables and supplementary data were not reviewed.
Source & supporting passages
Zhu K, Huang Z, Lv W, Liu Y, Lin Z, Wang Z, Xu H, Gu C, Sun S. · europepmc.org · September 21, 2026