Mouse protocol combining circadian disruption and high-fat, high-sugar diet
A protocol describes a C57BL/6J mouse model combining randomized circadian rhythm disruption with a high-fat, high-sugar diet; compared with single-factor control groups, the combined group showed more severe metabolic disorders, cognitive and motor impairments, and more significant accelerated aging. [1]
Automatic summary · not editorially reviewed
The key findings
The model combines randomized circadian rhythm disruption with a high-fat, high-sugar diet in C57BL/6J mice, with single-factor groups as controls. [1]
Compared with controls, the composite model showed more severe metabolic disorders, including increased body weight, elevated fasting glucose and lipid levels, and marked hepatic steatosis. [1]
The composite model also showed obvious cognitive and motor impairments and more significant accelerated aging, confirmed by the frailty index and hematoxylin and eosin staining. [1]
The source at a glance
- Study type / format
- Protocol describing an animal model study (abstract)
- Population / context
- C57BL/6J mice in a combined circadian disruption and high-fat, high-sugar diet model, with single-factor control groups.
- Summary based on
- Study abstract
- Original publication
- April 28, 2026
Combined lifestyle model in C57BL/6J mice
The protocol aims to establish a standardized mouse model that reflects multifactorial damage from modern, unhealthy lifestyles. The authors developed a C57BL/6J model combining randomized circadian rhythm disruption with a high-fat, high-sugar diet. Single-factor groups with only the high-fat, high-sugar diet and only circadian disruption served as controls.
Supporting source [1]
More severe metabolic and cognitive findings in composite group
Compared with controls, the composite model showed more severe metabolic disorders: increased body weight, elevated fasting glucose and lipid levels, and marked hepatic steatosis. It also showed obvious cognitive and motor impairments. The composite model exhibited more significant accelerated aging, confirmed by the frailty index and hematoxylin and eosin staining.
Supporting source [1]
Protocol scope: mouse model, not human evidence
The authors state that this integrated model reliably mimics lifestyle-related metabolic-cognitive decline and premature aging. The step-by-step reproducible protocol is intended to support mechanistic studies on lifestyle-mediated aging and the evaluation of targeted therapeutic strategies. These are findings in mice; the abstract does not report human outcomes or a tested therapy.
Supporting source [1]
How to interpret this
This is an abstract; the full text was not reviewed. The findings come from a mouse protocol, not human studies, and the abstract reports no human outcomes or tested therapy.
Based on the study abstract; the full paper, tables and supplementary data were not reviewed.
Source & supporting passages
Tu H, Kang T, Li H, Dong J, Cai J, Sun Q, Li Y, Li K, Zhao S, Li M. · europepmc.org · April 28, 2026