Niosome screening for gene delivery in Palbociclib-induced senescent UC-MSCs
In an abstract, two new niosome formulations were tested for gene delivery into Palbociclib-induced senescent umbilical cord stroma MSCs, with non-senescent cells as control; DP20CQ formulations gave the highest transgene expression in senescent cells. [1]
Automatic summary · not editorially reviewed
The key findings
Two new niosome formulations were tested on Palbociclib-induced senescent UC-MSCs, with non-senescent UC-MSCs as control. [1]
DP20CQ formulations outperformed DP80CH in both cell phenotypes from four donors, and at DOTMA/DNA ratios 10/1 and 15/1 gave the highest transgene expression in senescent cells, above Lipofectamine. [1]
DP20CQ formulations showed adequate physicochemical properties and marked endosomal escape, particularly at DOTMA/DNA 10/1, without compromising cell viability. [1]
The source at a glance
- Study type / format
- In vitro cell-culture study (abstract)
- Population / context
- Primary umbilical cord stroma mesenchymal stem cells (UC-MSCs) made senescent with Palbociclib, with non-senescent UC-MSCs as control, from four donors.
- Summary based on
- Study abstract
- Original publication
- October 8, 2026
Senescent UC-MSCs as gene-delivery target
The abstract frames niosome-based gene therapy as a possible way around off-target limits of first-generation senolytics, senomorphics and senoreverts. It reports testing two new niosome formulations on primary umbilical cord stroma mesenchymal stem cells (UC-MSCs) made senescent with Palbociclib, using non-senescent UC-MSCs as a control.
Supporting source [1]
DP20CQ versus DP80CH transfection results
Both formulations fixed the cationic lipid DOTMA and varied the non-ionic surfactant (polysorbate 20 or 80) and helper lipid (chloroquine or cholesterol), at two mole ratios each. In cells from four different donors, DP20CQ formulations outperformed DP80CH in both cell phenotypes. DP20CQ at DOTMA/DNA ratios of 10/1 and 15/1 gave the highest transgene expression in senescent UC-MSCs, above the commercial reagent Lipofectamine.
Supporting source [1]
Physicochemical properties and cell viability in the abstract
The abstract states that DP20CQ formulations had adequate physicochemical properties for transfection and promoted marked endosomal escape, particularly at a DOTMA/DNA ratio of 10/1, without compromising cell viability. It concludes that niosomes may be useful gene delivery vehicles for senescent cells in antiaging strategies. These are cell-culture findings from an abstract; the full text was not reviewed, and no human or lifespan benefit is shown.
Supporting source [1]
How to interpret this
Abstract only; the full text was not reviewed. Results come from cell-culture experiments with four donors and do not establish human, clinical or lifespan benefits.
Based on the study abstract; the full paper, tables and supplementary data were not reviewed.
Source & supporting passages
Iglesias-Fente A, López-Seijas J, Arufe MC, Fafián-Labora JA, Rey-Rico A. · europepmc.org · October 8, 2026