Multigenerational daytime-restricted feeding and liver DNA damage after irradiation in mice
In mice fed only during a 6-hour daytime window across generations F0–F10, the F10 generation showed disrupted circadian rhythms of DNA repair genes and, after 1 Gy X-ray irradiation, more DNA damage and senescent liver cells than earlier generations. [1]
Automatic summary · not editorially reviewed
The key findings
Mice from generations F0 to F10 were fed within a 6-hour window during the light phase (ZT2–ZT8). [1]
After 1 Gy X-ray irradiation, F10 mice showed significantly more γH2AX foci, indicating exacerbated DNA damage. [1]
F10 mice had higher p21 expression and more senescent cells, while apoptosis markers BAX and BCL2 were unchanged. [1]
The source at a glance
- Study type / format
- Preclinical mouse study (abstract)
- Population / context
- Mice from generations F0 to F10 in a multigenerational feeding model
- Summary based on
- Study abstract
- Original publication
- September 9, 2026
Mouse model of daytime-restricted feeding F0–F10
The study used a multigenerational mouse model in which animals from generation F0 to F10 had their feeding window restricted to 6 hours during the light phase (Zeitgeber time ZT2–ZT8). The authors analysed hepatic transcriptional rhythms and then exposed mice to 1 Gy X-ray irradiation to induce DNA damage. The stated aim was to examine intergenerational effects of this feeding schedule on the liver's DNA damage response and liver health.
Supporting source [1]
More DNA damage and senescence in F10 livers
Multigenerational daytime-restricted feeding disrupted circadian transcriptional rhythms of key DNA repair genes such as Wrnip1, Nabp2 and Fan1, with a trend towards downregulation in F10 livers. After irradiation, F10 mice had significantly more γH2AX foci, a marker of DNA damage, than earlier generations. They also showed higher p21 expression and more senescence-associated beta-galactosidase-positive cells, while apoptosis markers (BAX, BCL2) were unchanged.
Supporting source [1]
Mouse-only findings on feeding timing and liver stress
The authors conclude that persistent multigenerational daytime-restricted feeding impairs circadian regulation of DNA repair genes and sensitises the mouse liver to genotoxic stress, increasing DNA damage and cellular senescence. These are findings in mice exposed to irradiation, not evidence about human lifespan or human liver health. The abstract reports no sample sizes per generation, no follow-up details and no data beyond the described markers.
Supporting source [1]
How to interpret this
Abstract only; the full text was not reviewed. Findings come from a mouse model with irradiation and do not establish effects on human lifespan or liver health. Sample sizes, follow-up and detailed methods are not reported in the abstract.
Based on the study abstract; the full paper, tables and supplementary data were not reviewed.
Source & supporting passages
Sun L, Huang K, Cheng H, Wang Y, Yang X, Zhang W, Qian J, Zhai Q. · europepmc.org · September 9, 2026