Manganese ion in adult human dermal fibroblast cultures: proliferation and senescence markers
In cultured adult human dermal fibroblasts, 25 µmol/L manganese ion for 24–48 h was linked to more proliferation, fewer cells in G0/G1 and less SA-β-Gal positivity in most populations, with reduced PI3K/AKT signalling; extracellular matrix gene effects varied. [1]
Automatic summary · not editorially reviewed
The key findings
The study evaluated the anti-ageing potential of the manganese ion in cultures of adult human dermal fibroblasts treated with 25 µmol/L Mn for 24–48 h. [1]
Mn treatment enhanced proliferation and reduced the percentage of adult HDFs in G0/G1 and SA-β-Gal positivity in most populations. [1]
Mn reduced PI3K/AKT expression and activation, while extracellular matrix gene effects varied, with a trend towards more COL1A1 and less MMP1 in some populations. [1]
The source at a glance
- Study type / format
- In vitro cell-culture study (abstract)
- Population / context
- Cultured adult human dermal fibroblasts (HDFs)
- Summary based on
- Study abstract
- Original publication
- June 26, 2026
Manganese tested in adult skin fibroblast cultures
The study asked whether the manganese ion (Mn) has anti-ageing potential in cultures of adult human dermal fibroblasts (HDFs). The context given is that fibroblast proliferation declines during skin ageing, with reduced collagen turnover, more senescence markers and secretion of pro-inflammatory molecules linked to the senescence-associated secretory phenotype. The authors describe the PI3K/AKT/mTOR signalling axis as a central regulator of cell metabolism, growth and survival and note that its attenuation has been named among main anti-ageing interventions. HDFs were treated with 25 µmol/L Mn for 24–48 h.
Supporting source [1]
More proliferation, fewer G0/G1 cells, less SA-β-Gal
Proliferation was measured by tritiated thymidine incorporation, cell cycle progression by flow cytometry and senescence by SA-β-Gal staining. Mn treatment enhanced proliferation and reduced the percentage of adult HDFs in the G0/G1 phase and SA-β-Gal positivity in most HDF populations. PI3K/AKT pathway activity was measured by Western blot; Mn reduced its expression and activation. Extracellular matrix gene expression (COL1A1, MMP1) was measured by RT-qPCR and showed variable effects, with a trend towards increased COL1A1 and decreased MMP1 in some HDF populations.
Supporting source [1]
Cell-culture evidence, not human skin outcomes
The authors conclude that Mn may act as a bioactive ion influencing ageing-related pathways beyond its antioxidant properties, and that it may contribute to cellular responses associated with improved dermal function. These are findings in cultured adult HDFs, not measurements of human skin, lifespan or clinical outcomes. The abstract reports no sample sizes, donor numbers or statistical details, and the full text was not reviewed. The variable extracellular matrix effects and the trend-level changes in some populations limit how broadly the results can be generalised.
Supporting source [1]
How to interpret this
Abstract only; the full text was not reviewed. Findings come from cultured adult human dermal fibroblasts, not human skin or clinical outcomes. Sample sizes, donor numbers and statistical details are not reported in the abstract, and extracellular matrix effects varied between populations.
Based on the study abstract; the full paper, tables and supplementary data were not reviewed.
Source & supporting passages
Aranda D, Videla CMF, de Beláustegui E, Shayo C, Vellón L, Durand LAH. · europepmc.org · June 26, 2026